White Papers
18 December 2025
Optimizing AAV Serotype Filtration with Sartoclear Dynamics®️ Lab
In this application note we present data showcasing the clear filtration of different AAV serotypes using Sartoclear Dynamics®️ Lab in combination with the Sartolab®️ Multistation. Clarification experiments were first optimized in 250 mL batches and further upscaled to 1 L fractions. The production of the AAV material in HEK293 cells as well as the process optimization, analytical measurements and upscaling experiments were designed and performed by Ascend Advanced Therapies, a leading CDMO for high-quality, cost-effective gene and advanced therapy development and manufacturing. The further described results show that Sartoclear Dynamics®️ Lab can be used to simplify and accelerate the process of HEK lysate clarification and allows to reduce the hands-on time for clarification by a factor of ~6x compared to classical centrifugation followed by membrane-based clear filtration.
Download7 August 2025
In-Line Monitoring of CPPs and Capsid Titer in Upstream AAV Process with ProCellics™ Raman Analyzer
This application note explores the use of the ProCellics™ Raman Analyzer for in-line monitoring of critical process parameters (CPPs) during the growth and production phase as well as post-lysis capsid titer in the bioreactor of an AAV production process that utilizes Human Embryonic Kidney (HEK) cell culture and transient transfection.
Download7 August 2025
Optimizing AAV Serotype Filtration
In this application note we present data showcasing the clear filtration of different AAV serotypes using Sartoclear Dynamics® Lab in combination with the Sartolab® Multistation.
Download5 May 2025
Solving the AAV Scalability Challenge
See how our team worked with Sartorius to demonstrate consistent productivity, quality, and potency from 250 mL to 200 L scales.
Download